Test Name:
CXCR4 Mutation Analysis
CPT Code:
81479
Turn Around Time:
3 – 7 days
Clinical Application:
Detection of mutations within the c-terminus of the CXCR4 gene. Mutations are associated with resistance to ibrutinib.
Diagnosis.
Methodology:
Sanger sequencing. LoD: 10-20%
Optional: Cell sorting for increased sensitivity
Specimen Requirements:
DNA-based assays
Peripheral Blood: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)
Bone Marrow: Minimum 1 mL in EDTA (purple top) preferred or 1 mL in sodium heparin (green top)
Paraffin Sections: Minimum of 5 sections at 3 microns each
Note: FFPE bone marrow biopsies can result in inadequate DNA due to decalcification (clots sections preferable).
FFPE for Dermatology-related specimens are not accepted.
Storage and Transportation:
Please store and ship specimens at ambient temperatures.
Please refer to our full specimen requirements at Hematologics/Specimen Requirements for additional information and for directions on the storage and shipping of extracted DNA or RNA.