FLT3 ITD/TKD Mutation Analysis
PCR fragment analysis with EcoRV digest detecting FLT3 internal tandem duplications (ITD) and D835 tyrosine kinase domain mutations in AML.
IDH1 Mutation Analysis
Sanger sequencing of IDH1 exon 4 mutations including R132 to inform IDH inhibitor treatment decisions.
IDH2 Mutation Analysis
Sanger sequencing of IDH2 exon 4 mutations at codons R140 and R172 to inform IDH inhibitor treatment decisions.
ABL1 Tyrosine Kinase Domain Mutation Analysis
Sanger sequencing of ABL1 tyrosine kinase domain to detect imatinib resistance mutations in BCR::ABL1-positive CML, ALL, and AML.
CD33 SNP Genotyping
Allele-specific PCR genotyping of the CD33 rs12459419 SNP, associated with CD33 antigen expression and gemtuzumab response.
CXCR4 Mutation Analysis
Sanger sequencing of CXCR4 c-terminal mutations associated with ibrutinib resistance in Waldenström's macroglobulinemia.