Test Name:
FLT3 ITD/TKD Mutation Analysis
CPT Code:
81245
Turn Around Time:
24 – 72 hours
Clinical Application:
Detection of FLT3 internal tandem duplications (ITD) and missense mutation affecting codon D835 within the tyrosine kinase domain (TKD) of FLT3.
Diagnosis and monitoring.
Methodology:
PCR, fragment length analysis and EcoRV restriction enzyme digest. LoD: 1-5%
Tyrosine kinase domain mutations other than in codon 835 are not detected.
For sensitive monitoring purposes, deep sequencing (NGS) is required.
Rush testing is available on request.
Specimen Requirements:
DNA-based assays
Peripheral Blood: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)
Bone Marrow: Minimum 1 mL in EDTA (purple top) preferred or 1 mL in sodium heparin (green top)
Paraffin Sections: Minimum of 5 sections at 3 microns each
Note: FFPE bone marrow biopsies can result in inadequate DNA due to decalcification (clots sections preferable).
FFPE for Dermatology-related specimens are not accepted.
FFPE is not an acceptable sample for NGS-based assays.
Storage and Transportation:
Please store and ship specimens at ambient temperatures.
Please refer to our full specimen requirements at Hematologics/Specimen Requirements for additional information and for directions on the storage and shipping of extracted DNA or RNA.