Test Name:

WT1 Gene Transcript Levels by RT-PCR

CPT Code:

81479

Turn Around Time:

24 – 72 hours

Clinical Application:

Test quantitatively determines Wilm’s Tumor (WT1) transcript levels, for monitoring of MDS and AML.

Diagnosis and monitoring.

Methodology:

Upper remission limit 0.01. WT-1 expression levels in patients in permanent remission may remain detectable by RT-PCR.

WT-1 expression transcripts in patients in permanent remission may remain detectable by RT-PCR.

Specimen Requirements:

RNA-based assays

Peripheral Blood: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)

Bone Marrow: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)

Storage and Transportation:

Please store and ship specimens at ambient temperatures.

Ship RNA-based assays within 24 hours for optimal results.

Please refer to our full specimen requirements at Hematologics/Specimen Requirements for additional information and for directions on the storage and shipping of extracted DNA or RNA.