Test Name:
T-Cell Receptor Beta Gene Rearrangement (TCRB)
CPT Code:
81340
Turn Around Time:
24 – 72 hours
Clinical Application:
Detection of clonal T-Cell receptor beta rearrangements by PCR.
Diagnostic and disease monitoring to the level of sensitivity.
Methodology:
PCR, fragment length analysis, LoD: 1-10%
Dermatology samples not accepted. Test may be ordered concurrently with T-Cell receptor gamma gene rearrangement studies, or as a reflex when TCRG testing is negative.
Specimen Requirements:
DNA-based assays
Peripheral Blood: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)
Bone Marrow: Minimum 1 mL in EDTA (purple top) preferred or 1 mL in sodium heparin (green top)
Paraffin Sections: Minimum of 5 sections at 3 microns each
Note: FFPE bone marrow biopsies can result in inadequate DNA due to decalcification (clots sections preferable).
FFPE for Dermatology-related specimens are not accepted.
FFPE is not an acceptable sample for NGS-based assays.
Storage and Transportation:
Please store and ship specimens at ambient temperatures.
Please refer to our full specimen requirements at Hematologics/Specimen Requirements for additional information and for directions on the storage and shipping of extracted DNA or RNA.