Test Name:
JAK2 V617F Mutation Analysis
CPT Code:
81270
Turn Around Time:
24 – 72 hours
Clinical Application:
Detection of the JAK2 p.V617F mutation, associated with myeloproliferative neoplasm.
Diagnosis and monitoring.
Methodology:
Allele specific (AS) PCR. Minimum sensitivity: 0.1%
Optional: quantitative
Specimen Requirements:
DNA-based assays
Peripheral Blood: Minimum 2 mL in EDTA (purple top) preferred or 2 mL in sodium heparin (green top)
Bone Marrow: Minimum 1 mL in EDTA (purple top) preferred or 1 mL in sodium heparin (green top)
Paraffin Sections: Minimum of 5 sections at 3 microns each
Note: FFPE bone marrow biopsies can result in inadequate DNA due to decalcification (clots sections preferable).
FFPE for Dermatology-related specimens are not accepted.
Storage and Transportation:
Please store and ship specimens at ambient temperatures.
Please refer to our full specimen requirements at Hematologics/Specimen Requirements for additional information and for directions on the storage and shipping of extracted DNA or RNA.